An accidential over a trill sign is applied to the auxilliary note of the trill (the note above the main note written). The auxillary note is usually one LETTER above the main. Eg: If you have a written C, aux is D. The auxillary, usually follows the key signature, or the immediate harmony of which it is in context. If there is to be any deviation from this it is usually written as an accidental above or beside the trill sign. In your case: Written Eb trills with some sort of F. Because there is a flat over the trill you trill Eb to Fb.