There is one important thing missed in this thread. It is the fact that when measuring frequencies we are referring to pitch levels.
A440 means that A beats @ 440 cycles per second. This is what is called International Standard Pitch which was agreed to in 1939 at an international conference. This technical standard was taken up by the International Organization for Standardization in 1955 and reaffirmed by them in 1975 as ISO 16.
Interesting to note that the original standard was 439, but there were complaints about this. The “diapason normal” varied from 440 and the culprit was thought to be the temperature the French took the measurement at. The other complaint about the French using 439 was that 439 is a prime number.
So back to the OP question. I believe the assignment has been misunderstood because of a lack of knowledge about music, musicology, and what defines pitch.
The answer would be once A440 varies from 440cps it is out of tune according to the international standard frequency.
Now it may not be out if tune with the rest of the instrument but that is not what is being asked.
The first sentence of the OP reads:
Hello, I'm currently on an assignment where I'm needed to analyse the frequency spectrum of a piano note...(snip)
The OP goes on to state that he/she has found the frequencies for other notes. This has nothing to do with being in tune or out of tune.
It has to do with the measurement of pitch, which is a frequency cycle.